Design bRNAs across a locus

Design all possible bRNAs targeting a given locus.

Target Locus Name

bRNA Scaffold

Target Locus Sequence

Donor Name

Donor Sequence

Cores (e.g. CT, GT) Comma-separated list

Check for Off-Targets

Genbank Files to Screen Off-targets

kmer Length Matches are assessed based on the first X bp.

Levenshtein Distances to Calculate Allow for mismatches in off-target analysis

Score Predicted Secondary Structure

Avoid Restriction Sites Comma-separated list

Primer Seqs (Dict Format)